Abstract

 Aim and Objectives: This project aims to study the role of the Novel Autologous Platelet Rich Plasma Therapy Protocol in endometrial preparation for ART and to evaluate the implantation rate, Clinical pregnancy rate and Outcome of pregnancy in patients with successful implantation.

Introduction: Embryo–endometrial crosstalk is a prerequisite for successful implantation and pregnancy. The concept of the Window of Implantation (WOI) and endometrial receptivity is still a grey area due to a lack of knowledge regarding its exact role in infertility and implantation failure. The pregnancy rate is seen to be proportional to an increase in endometrial thickness. The use of Platelet-rich Plasma (PRP) in Assisted Reproductive Techniques (ART) is growing, attributable to its ability to promote endometrial growth and receptivity. Platelet-rich plasma (PRP) is 4–5 times richer in platelets than circulating blood. By activating platelets in PRP, cytokines and growth factors (GFs) are activated, which regulate cell migration, attachment, proliferation and differentiation and promote extracellular matrix accumulation. In the present study, we have administered the novel autologous intrauterine PRP therapy during the proliferative phase of the endometrium, that is on Day 5, Day 10 and 48 hours before embryo transfer (ET).

Results: All 32 patients participating in the study received 3 fixed doses of PRP therapy on Day 5, Day 10 and at 48 hours before Embryo Transfer. 34.37% of the women showed an endometrial thickness between 7 to 8 mm whereas 65.63% showed an endometrial thickness between 8 to 9mm post therapy. The overall clinical pregnancy rate was 75%. Implantation rate was 76.19% (8 twin gestation and 16 singleton). 26.19% of pregnancies were carried to term and resulted in successful delivery. PRP infusion resulted in maximum clinical pregnancy rates and pregnancies carried to term in women belonging to the 30-35 yrs age group.

Discussion and Conclusion: Optimal endometrium thickness is a critical factor for successful embryo implantation. Consequently endometrial preparation has been considered a crucial step for embryo transfer.